Specifications

• Conjugation:
  Biotin
• Protein/Peptide Type:
  Recombinant
• Species:
  Human
• Tag sequence:
  Fc, Avitag™
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) with a linear range of 1-39 ng/mL (QC tested). Measured by its binding ability in a functional ELISA. Immobilized Human IL-2 R alpha, Fc Tag (Cat. No. ILA-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) with a linear range of 0.6-10 ng/mL (Routinely tested). Loaded Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) on SA Biosensor, can bind Human IL-2 R beta, His Tag (SPR verified) (Cat. No. CD2-H5221) with an affinity constant of 2.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) on SA Biosensor, can bind Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) with an affinity constant of 47.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  P60568-1
• Protein Synonyms:
  TCGF|lymphokine|IL2|Interleukin 2
• Protein/Peptide Name:
  IL-2
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular Weight:
  43.7 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT