Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Species:
  Human
• Size:
  1 mg
• Tag sequence:
  His Tag&Strep II Tag
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Measured by its binding ability in a functional ELISA. Immobilized Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag (Cat. No. ILF-H52WF) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) with a linear range of 0.039-0.625 μg/mL (QC tested). Measured by its binding ability in a functional ELISA. Immobilized Anti-IL17A MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag (Cat. No. ILF-H52WF) with a linear range of 0.2-3 ng/mL (Routinely tested). Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag (Cat. No. ILF-H52WF) with an affinity constant of 34.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag (Cat. No. ILF-H52WF) stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 3.14-9.01 ng/mL (Routinely tested).
• Gene ID:
  Q16552-1 (IL-17A) & Q96PD4-1 (IL-17F)
• Protein Synonyms:
  IL-17|IL-17F|IL-17A|Interleukin-17F|CTLA-8|Cytokine ML-1|Interleukin-17A
• Protein/Peptide Name:
  IL-17A & IL-17F
• Purity:
  >95% as determined by reduced SDS-PAGE.
• Molecular Weight:
  17.0 kDa (IL-17A) & 18.4 kDa (IL-17F)
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT
• Cat. No.:
  103790-584