Specifications

• Conjugation:
  Biotin
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  His, Avitag™
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Human FGL1, His,Avitag (recommended for biopanning) (Cat. No. FG1-H82Ey) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Anti-FGL1 antibody with a linear range of 0.01-0.625 μg/mL (QC tested). Loaded Biotinylated Human FGL1, His,Avitag (recommended for biopanning) (Cat. No. FG1-H82Ey) on SA Biosensor, can bind Human LAG-3, Fc Tag (HPLC-verified) (Cat. No. LA3-H5255) with an affinity constant of 8.12 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). FACS assay shows that Biotinylated Human FGL1, His,Avitag (Cat. No. FG1-H82Ey) can bind to 293T cells overexpressing Human LAG3. The concentration of Human FGL1 is 10 μg/mL (Routinely tested).
• Gene ID:
  Q08830-1
• Protein Synonyms:
  Hepassocin|HP-041|HFREP1|FGL1|LFIRE-1|HFREP-1
• Protein/Peptide Name:
  FGL1
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular Weight:
  37.9 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS with Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT