Specifications

• Conjugation:
  Biotin
• Protein/Peptide Type:
  Recombinant
• Species:
  Human
• Tag sequence:
  His, Avitag™
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) captured on Biotin CAP - Series S sensor Chip can bind Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with an affinity constant of 22.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-C82W6) on SA Biosensor, can bind Human CD3×BCMA scFV with an affinity constant of 1.84 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  P07766-1 (CD3E) & P04234-1 (CD3D)
• Protein Synonyms:
  CD3 delta / epsilon|CD3 delta & CD3 epsilon|CD3E & CD3D
• Protein/Peptide Name:
  CD3E & CD3D
• Purity:
  >90% as determined by SDS-PAGE.
• Molecular Weight:
  18.6 kDa (CD3E) and 13.4 kDa (CD3D)
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT