Cynomolgus Monkey Recombinant Siglec-15 (from HEK293 cells)

About this item

Cynomolgus Siglec-15 / CD33L3 Protein, Fc Tag, ACROBiosystems

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Specifications

Conjugation:
Unconjugated
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Cynomolgus monkey
Tag sequence:
Fc Tag
Storage Conditions:
–20 °C
Endotoxin Content:
Less than 1.0 EU per μg by the LAL method.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus Siglec-15, Fc Tag (Cat. No. SG5-C5253) at 5 μg/mL (100 μL/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested). Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus Siglec-15, Fc Tag (Cat. No. SG5-C5253) at 10 μg/mL (100 μL/well) on Diamond Protein A Protein, His Tag precoated (0.5 μg/well) plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.078-1.25 μg/mL (Routinely tested). Loaded Cynomolgus Siglec-15, Fc Tag (Cat. No. SG5-C5253) on ProteinA Biosensor, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with an affinity constant of 0.62 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Gene ID:
A0A2K5UY47-1
Protein Synonyms:
Siglec-15|SIGLEC-15|Siglec15|CD33L3|CD33 antigen-like 3|sialic acid-binding Ig-like lectin 15
Protein/Peptide Name:
Siglec-15
Purity:
>95% as determined by SDS-PAGE.
Molecular Weight:
52.7 kDa
Endotoxin Level:
Low
Formulation:
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Shipping Temperature:
RT