AMV RT catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids.

• For sequencing, mutagenesis, cDNA synthesis, cDNA cloning and primer extension
• Efficient Reverse Transcription of Templates with High Secondary Structure
• DNA polymerization using template DNA, RNA or RNA:DNA hybrids
• Requires a primer as well as magnesium or manganese
• Stable at higher reaction temperatures (37-58°C)
• Supplied at a concentration of 10u/microl (Cat.# M5101, M5108) or 20-25u/µl (Cat.# M9004)

Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg(2+) or Mn(2+). The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.