Klenow Fragment (3'-5' exo-)

Klenow Fragment (3´→ 5´ exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has mutations (D355A, E357A) which abolish the 3´→ 5´ exonuclease activity.

Enzyme Name:
Klenow Fragment (3'-5' exo-)
Enzyme Activity:
5000 units/ml

New England BioLabs Molecular Cloning Technical Guide

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