igScript™ Probe-Based RT-qPCR Kit provides improved PCR efficiency, wider dynamic range, superior sensitivity and specificity.

• Enhanced efficiency, specificity, and sensitivity
• Compatible with all real-time PCR instruments
• Superior gene expression results under various cycling conditions
• Robust and active for cDNA synthesis at temperatures up to 50 °C

IgScript™ first strand cDNA synthesis kit includes 5× IgScript™ master mix which contains IgScript™ Reverse Transcriptase, RNase inhibitor, dNTPs, an optimized buffer, MgCl₂ and protein stabilizers. IgScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit also provides two optimized primers and nuclease free water. An anchored Oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail and the random hexamer primer mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42 to 50 °C.

Probe-based qPCR 2× master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA. This 2× master mix includes proprietary buffer, Taq DNA polymerase, low ROX reference dye, MgCl₂, dNTPs, stabilizers and enhancers.