Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
  Mouse
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Measured by its binding ability in a functional ELISA. Immobilized Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) at 10 μg/mL (100 μL/well) can bind Human IL-23A and Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) with a linear range of 0.039-0.313 μg/mL (QC tested).; Loaded Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) on Protein A Biosensor, can bind Human IL-23A and Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) with an affinity constant of 10.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) on Protein A Biosensor, can bind Human IL-23A and Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) with an affinity constant of 2.79 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; Human IL-23A and Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) stimulates secretion of IL-17 by Mouse spleen cells. The ED50 for this effect is 0.0069-0.0426 ng/mL (Routinely tested).; Cell based assay shows that the secretion of IL-17 induced by Human IL-23A and Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) is inhibited by increasing concentration of the Anti-Human IL-23 neutralizing antibody. The IC50 is between 0.41-1.012 ng/mL (Routinely tested).
• Gene ID:
  Q9NPF7-1 (IL23A) and P43432-1 (IL12B)
• Carrier-Free:
  Y
• Protease-free:
  Y
• Animal-Free:
  Y
• Protein Synonyms:
  IL-23 alpha and IL-12 beta
• Protein/Peptide Name:
  IL23A and IL12B
• Purity:
  >95%
• Molecular Weight:
  19.5 kDa (IL23A) and 35.8 kDa (IL12B)
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Nuclease-free:
  Y
• Shipping Temperature:
  Powder,RT