Human Recombinant TSLP R (from HEK293)

About this item

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Specifications

Conjugation:
Unconjugated
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Human
Storage Conditions:
–20 °C
Endotoxin Content:
Less than 1.0 EU per μg by the LAL method.
Biological Activity:
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) with a linear range of 4-31 ng/mL (QC tested).; Measured by its binding ability in a functional ELISA. Biotinylated Human TSLP (R127A, R130A), His,Avitag (Cat. No. TSP-H82E0) immobilized at 2 μg/mL (100 μL/well) via precoated 5 μg/mL (100 μL/well) of Human TSLP R, Fc Tag (Cat. No. TSR-H525a), can bind can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) with a linear range of 1-20 ng/mL (Routinely tested).; Captured Human TSLP, His Tag (Cat. No. TSP-H52Hb) on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 1.81 nM as determined in SPR assay (Biacore T200) (Routinely tested).; Captured Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 4.22 nM as determined in SPR assay (Biacore T200) (Routinely tested).; Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) with an affinity constant of 16 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Gene ID:
Q9HC73-1
Carrier-Free:
Y
Protease-free:
Y
Animal-Free:
Y
Protein Synonyms:
TSLP receptor|CRL2|TSLPR|Cytokine receptor-like 2|ILXR|p2RY8/CRLF2 fusion|TSLP R|CRLF2|IL-XR|CRLF2Y
Protein/Peptide Name:
TSLP R
Purity:
>95%
Molecular Weight:
50.4 kDa
Endotoxin Level:
Low
Formulation:
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Nuclease-free:
Y
Shipping Temperature:
Powder,RT