Human Recombinant Siglec-3 (from HEK293)
- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:HEK293 cells
- Species:Human
- Storage Conditions:–20 °C
- Endotoxin Content:Less than 0.01 EU per μg by the LAL method.
- Biological Activity:Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin (Cat. No. CD3-H5259) at 5 μg/mL (100 μL/well) can bind Anti-CD33, Human IgG1 with a linear range of 0.078-1.25 μg/mL (QC tested).; Loaded Siglec-3 MAb (Mouse IgG1) on AMC Biosensor, can bind Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin (Cat. No. CD3-H5259) with an affinity constant of 0.365 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- Gene ID:P20138-1(R69G)
- Carrier-Free:Y
- Protease-free:Y
- Animal-Free:Y
- Protein Synonyms:gp67|SIGLEC3|CD33
- Protein/Peptide Name:Siglec-3
- Purity:>95%
- Molecular Weight:54.6 kDa
- Endotoxin Level:Low
- Formulation:Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
- Nuclease-free:Y
- Shipping Temperature:Powder,RT
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