Specifications

• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage Conditions:
  –20 °C, aviod light
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Monoclonal Anti-Human CD22 Antibody, Human IgG1 at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Siglec-2, His Tag (Cat. No. SI2-HF2H6) with a linear range of 0.078-5 μg/mL (QC tested).; 293 cells were transfected with anti-CD22-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human Siglec-2, His Tag (Cat. No. SI2-HF2H6, 10 µg/mL) and C. FITC-labeled Protein Control. A. Non-transfected 293 cells and C. FITC-labeled Protein Control were used as negative control. RFP was used to evaluate CAR (anti-CD22-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human Siglec-2, His Tag (Cat. No. SI2-HF2H6) (QC tested).
• Gene ID:
  P20273-1
• Carrier-Free:
  Y
• Protease-free:
  Y
• Animal-Free:
  Y
• Protein Synonyms:
  BL-CAM|SIGLEC-2|CD22|Siglec2|SIGLEC2|SIGLEC2FLJ22814
• Protein/Peptide Name:
  Siglec-2
• Purity:
  >95%
• Molecular Weight:
  77.0 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Nuclease-free:
  Y
• Shipping Temperature:
  Powder,RT, Avoid light