Human Recombinant RANK (from HEK293)
- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:HEK293 cells
- Species:Human
- Storage Conditions:–20 °C
- Endotoxin Content:Less than 0.1 EU per μg by the LAL method.
- Biological Activity:Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF11, His Tag (Cat. No. RAL-H5240) at 2 μg/mL (100 μL/well) can bind Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) with a linear range of 0.4-13 ng/mL (QC tested).; Captured Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) on CM5 chip via Anti-Mouse antibodies surface can bind Human TNFSF11, His Tag (Cat. No. RAL-H5240) with an affinity constant of 0.309 nM as determined in a SPR assay (Biacore T200) (Routinely tested).; Loaded Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) on AMC Biosensor, can bind Human TNFSF11, His Tag (active trimer) (Cat. No. RAL-H5240) with an affinity constant of 0.409 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- Gene ID:Q9Y6Q6-1
- Carrier-Free:Y
- Protease-free:Y
- Animal-Free:Y
- Protein Synonyms:CD265 antigen|TNFRSF11A|ODFR|ODFROSTS|RANK|OFE|OSTS|FEO|LOH18CR1|OPTB7|RANKLOH18CR1|CD265|PDB2|TRANCER|NFKB activator
- Protein/Peptide Name:RANK
- Purity:>95%
- Molecular Weight:47.2 kDa
- Endotoxin Level:Low
- Formulation:Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
- Nuclease-free:Y
- Shipping Temperature:Powder,RT
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