Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Size:
  1 mg
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 0.01 EU per μg by the LAL method.
• Biological Activity:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human PD-1, His Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H522a) at 2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5) with a linear range of 10-156 ng/mL (Routinely tested).; Loaded Human PD-1, His Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H522a) on HIS1K Biosensor, can bind Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) with an affinity constant of 23.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).; Loaded Human PD-1, His Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H522a) on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC-verified) (Cat. No. PD2-H5251) with an affinity constant of 15.7 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).; Loaded Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) on Protein A Biosensor, can bind Human Human PD-1, His Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H522a) with an affinity constant of 4.8 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; Loaded Human PD-L2, Fc Tag (HPLC verified) (Cat. No. PD2-H5251) on Protein A Biosensor, can bind Human Human PD-1, His Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H522a) with an affinity constant of 0.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  Q15116
• Carrier-Free:
  Y
• Protease-free:
  Y
• Animal-Free:
  Y
• Protein Synonyms:
  CD279|PDCD1|PD1|SLEB2
• Protein/Peptide Name:
  PD-1
• Purity:
  >95%
• Molecular Weight:
  16.8 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Nuclease-free:
  Y
• Shipping Temperature:
  Powder,RT
• Cat. No.:
  103738-006