Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; FMC63 MAb (mouse lgG2a) captured on CM5 chip via anti-mouse antibodies surface, can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with an affinity constant of 2.95 nM as determined in a SPR assay (Biacore T200) (Routinely tested).; Captured Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) on CM5 Chip via anti-His antibody, can bind FMC63 (mouse lgG2a) with an affinity constant of 2.01 nM as determined in SPR assay (Biacore T200 ) (Routinely tested).; Loaded FMC63 MAb (mouse lgG2a) on Protein A Biosensor, can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with an affinity constant of 4.36 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; Loaded Anti-CD19 MAb (Human IgG1) on AHC Biosensor, can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with an affinity constant of 5.41 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; 293 cells were transfected with FMC63-scFv and RFP tag. 2e5 of the cells were first incubated with A. Human His Tag control. B. Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2, 10 μg/mL). C. Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2, 10 μg/mL) and FMC63 (Mouse anti-CD19 antibody). The FITC Anti-6xHis tag antibody was used to analyse with FACS. RFP was used to evaluate CAR (FMC63-scFv) expression and FITC was used to evaluate the binding activity of Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2).
• Gene ID:
  P15391-1
• Carrier-Free:
  Y
• Protease-free:
  Y
• Animal-Free:
  Y
• Protein Synonyms:
  CVID3|MGC12802|B4|CD19
• Protein/Peptide Name:
  CD19
• Purity:
  >90%
• Molecular Weight:
  32.0 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Nuclease-free:
  Y
• Shipping Temperature:
  Powder,RT