Human Recombinant CD155 (from HEK293)

About this item

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Specifications

Conjugation:
Unconjugated
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Human
Storage Conditions:
–20 °C
Endotoxin Content:
Less than 0.1 EU per μg by the LAL method.
Biological Activity:
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT, Fc Tag (Cat. No. TIT-H5254) at 2 μg/mL (100 μL/well) can bind Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) with a linear range of 0.1-4 ng/mL (QC tested).; Measured by its binding ability in a functional ELISA. Immobilized Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) at 2 μg/mL (100 μL/well) can bind Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) with a linear range of 1-16 ng/mL (Routinely tested).; Serial dilutions of Human TIGIT Neutralizing antibody were added into Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254): Biotinylated Human TIGIT, Fc,Avitag (Cat. No. TIT-H82F1) binding reactions. The half maximal inhibitory concentration (IC50) is 0.08116 μg/mL (Routinely tested).; Loaded Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) on Protein A Biosensor, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H3) with an affinity constant of 0.24 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; Loaded Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) on Protein A Biosensor, can bind Human DNAM-1, His Tag (Cat. No. DN1-H52H6) with an affinity constant of 0.57 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; FACS assay shows that Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 μg/mL (Routinely tested).; FACS analysis shows that the binding of Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 μg/mL. The IC50 is 0.1413 μg/mL (Routinely tested).
Gene ID:
P15151-1 (I340M)
Carrier-Free:
Y
Protease-free:
Y
Animal-Free:
Y
Protein Synonyms:
TAGE4|PVS|FLJ25946|CD155|NECL5|PVR|HVED
Protein/Peptide Name:
CD155
Purity:
>95%
Molecular Weight:
62.2 kDa
Endotoxin Level:
Low
Formulation:
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Nuclease-free:
Y
Shipping Temperature:
Powder,RT