Quantitative determination of ferric reduction antioxidant potential (FRAP).

• Sensitive and accurate: linear detection range - 0.5 to 180 µm Fe³⁺ reduction potential in 96-well plate assay
• Method: OD 590 nm
• Samples: plant extracts, foods, vitamins, supplements, and biological samples such as serum, plasma, and urine

Ferric reduction antioxidant potential (FRAP) is a measure of antioxidant capacity quantified by the antioxidants potential to reduce ferric iron (III) to ferrous iron (II). Antioxidants protect cells from damage by reactive oxygen species that are produced by oxidation reactions. As oxidative stress contributes to the development of many diseases including Alzheimer's disease, Parkinson's disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and as preservatives in a wide range of products such as food, cosmetics, rubber and gasoline.

Simple, direct and high-throughput assays for antioxidant capacity find wide applications in research, food industry and drug discovery. BioAssay Systems improved assay measures antioxidant potential in which Fe³⁺ is reduced by antioxidant to Fe²⁺. The resulting Fe²⁺ specifically forms a colored complex with a chromogen. The color intensity at 590 nm is proportional to FRAP in the sample.