Our suite of specialized media provides our customers with a diverse set of tools to enhance research through consistent and stable environments for cell cultures. Cell Systems media may be used with all primary, immortalized, and established cell lines.

• Must be supplemented with 5 to 20% fetal bovine serum to become complete
• Do not contain serum
• Do not contain phenol red
• Packaged in 500 ml bottles (as a kit)
• Designed to meet your custom needs

Kit consists of 500 ml Cell Systems Normal Glucose Medium [No Serum] (glucose concentration = 0.9008 gL or 5 mM), 10 ml Attachment Factor™ (extracellular matrix reagent for coating flasks) and 5 ml CultureBoost™. This medium has been formulated at normal human plasma glucose level and becomes a complete medium with the addition of 5 to 20% serum (not included) and animal-derived growth factor (CultureBoost™, included). 4N3-500 is Certified and intended for experimental application.

Cell Systems Medium contains no added hormones. Medium contains no antibiotics; however, Bac-Off®, (Cat #4Z0-643), is the recommended antibiotic for all Cell Systems medium. Bac-Off® is used at or above the 2 µg/ml MIC of aerobic gram-positive and gram-negative microorganisms.

Medium contains no phenol red. Phenol red in cell culture medium binds to receptors on cell surfaces and causes the cell to respond as though pharmacologic levels of estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) are present in the medium. We exclude this unnecessary additive in order to optimize experimental conditions for researchers.

Certified for use with more than 100 human and animal cell cultures and cell lines including:
Human: All Cell Systems Primary Cells; Bone Marrow Cells (CFU-F and Erythroid Burst Cultures); Connective Tissue Cells; Dermal Fibroblast Cells; Endothelial Cells (Aortic, Arterial, Coronary Artery, Vascular and Venous); Fetal Lung Cells; Embryonic and Adult Stem Cells; Fibroblast Cells; Glomerular Mesangial Cells; Immortalized / Tumor-derived Mesenchymal Cells; Microvascular Cells (Cerebral, Coronary, Dermal, Glomerular, Liver, Lung and Retinal); Smooth Muscle Cells; Tumor Neovascular Cells.

Medium Storage: Cell Systems Medium is shipped at ambient temperature. If the entire unit will not be used within 30 days, supplement the medium with serum (not included) and CultureBoost™, then aliquot and freeze in smaller units which will be used within 30 days. Otherwise, immediately refrigerate the medium and supplement. Once the unit is supplemented, or any component of the medium kit is opened, the shelf life is 30 days at refrigerated (2 to 8 °C) temperatures.
For best results, use Cell Systems Medium in conjunction with cell culture vessels coated with Attachment Factor™ extracellular matrix reagent.