Our suite of specialized media provides our customers with a diverse set of tools to enhance research through consistent and stable environments for cell cultures. Cell Systems media may be used with all primary, immortalized, and established cell lines.

• Based on a modified and optimized basal DMEM/F12 (1:1)
• Contain serum at 10% by volume
• Do not contain phenol red
• Packaged in 500mL bottles (as a kit)

Medium is formulated with 10% serum. Kit consists of 500 ml 4N0-500 Cell Systems normal glucose medium (glucose concentration = 0.9008 g/L or 5mM), 5 ml CultureBoost™ (containing animal derived growth factors) and 10 ml Attachment Factor™ (extracellular matrix reagent for coating flasks). This growth medium has been formulated at normal human plasma glucose level and becomes a complete medium once activated with the included CultureBoost™ supplement. 4N0-500 is Certified and intended for experimental application.

Cell Systems Medium contains no phenol red.Phenol red in cell culture medium binds to receptors on cell surfaces and causes the cell to respond as though pharmacologic levels of estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) are present in the medium. We exclude this unnecessary additive in order to optimize experimental conditions for researchers.

Cell Systems Medium contains no antibiotics; however, Bac-Off® (Cat. #4Z0-643), is the recommended antibiotic for all Cell Systems media. Bac-Off® is used at or above the 2 µg/ml MIC of aerobic Gram-positive and Gram-negative microorganisms.

Certified for use with more than 100 human and animal cell cultures and cell lines including:
Human: All Cell Systems Primary Cells; Bone Marrow Cells (CFU-F and Erythroid Burst Cultures); Connective Tissue Cells; Dermal Fibroblast Cells; Endothelial Cells ( Aortic, Arterial, Coronary Artery, Vascular and Venous); Fetal Lung Cells; Embryonic and Adult Stem Cells; Fibroblast Cells; Glomerular Mesangial Cells; Immortalized / Tumor-derived Mesenchymal Cells; Microvascular Cells (Cerebral, Coronary, Dermal, Glomerular, Liver, Lung and Retinal); Smooth Muscle Cells; Tumor Neovascular Cells.

Medium Storage: Cell Systems Medium is shipped at ambient temperature. If the entire unit will not be used within 30 days, supplement the medium with CultureBoost™, then aliquot and freeze in smaller units which will be used within 30 days. Otherwise, immediately refrigerate the medium and supplement. Once the unit is supplemented, or any component of the medium kit is opened, the shelf life is 30 days at refrigerated (2 to 8°C) temperatures.
For best results, use Cell Systems Medium in conjunction with cell culture vessels coated with Attachment Factor™ extracellular matrix reagent.