Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. Biotin-dUTP conjugates are available wtih 11-carbon, 16-carbon, or 20-carbon linker lengths.

• 16-carbon linker between biotin and dUTP
• Available in solid or solution form
• Solid contains lyophilized TE buffer
• Solution is 1 mM in Tris-HCl buffer pH 7.5

In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. On the other hand, a longer linker should facilitate interaction between biotin and avidin or streptavidin.