Preservative-free Annexin V conjugates are compatible with detection of apoptotic cells in live cell imaging experiments, fluorescence microscopy, or flow cytometry.

• Azide-free for no-wash, real-time live cell imaging in culture medium
• Fast, reliable detection of phosphatidylserine on apoptotic cells
• Choice of 11 CF® dye colors with superior brightness and photostability
• For real-time live cell imaging, fluorescence microscopy, or flow cytometry

CF® Dye Annexin V Conjugates can be used to stain the surface of apoptotic cells. The human anticoagulant Annexin V is a 35 to 36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.

These conjugates are supplied as lyophilized solids with no azide or other preservatives that might be incompatible with live cell imaging. After reconstitution in buffer, the conjugates can be added to cell culture medium for no-wash, real-time live cell imaging. Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes.

Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.