Firefly Luciferase Assay Kit 2.0, Biotium

Supplier: Biotium

Supplier: Biotium

About this item

This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.

Highly sensitive and linear flash-type assay
Substrate is added fresh every assay, for consistent signal over time
Excellent performance and great value compared to other suppliers

This firefly luciferase assay kit is designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. Other commercial luciferase assays require you to dilute all of the firefly substrate in the assay buffer, resulting in limited shelf life after first use. In Biotium’s kits, the substrates are provided as separate stock solutions that are freshly diluted for every assay, for excellent shelf life with consistent signal over time.

Biotium also offers the Firefly and Renilla Luciferase Single Tube Assay Kit (75845-708), a combined luciferase assay kit for sequential measurement of Firefly and Renilla luciferase activity sequentially in the same sample with high sensitivity and linearity.

Ordering information: Assay size is based on 96-well format. The kits include enough cell lysis buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. Additional 5X Firefly Lysis Buffer (89410-734) can be purchased separately if needed.

Delivery: Kit components include 5X Firefly Luciferase Lysis Buffer, Firefly Luciferase Assay Buffer 2.0 and D-Luciferin.

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