This kit is designed for sequential measurement of Firefly and Renilla luciferase activities in transfected cell reporter assays.

• Sequentially measure firefly and Renilla activities in a single sample
• Highly sensitive and linear flash-type assay
• Easy-to-handle water-soluble substrates
• Substrate is added fresh every assay, for consistent signal over tim
• Performs as well as leading brands, at a lower cost

The Firefly and Renilla Luciferase Single Tube Assay is a combined luciferase assay allowing sequential measurement of Firefly and Renilla luciferase activity in the same sample with high sensitivity and linearity. Firefly luciferase activity is measured first, then Renilla Luciferase Assay Buffer 2.0 is added to the sample to simultaneously quench firefly luciferase activity and measure Renilla luciferase activity. Note: This is a flash-type assay that requires luminescence to be measured immediately after adding luciferase detection reagents to your sample. It requires a single-tube luminometer or luminescence microplate reader with reagent injectors.

Other commercial luciferase assays require you to dilute all of the firefly substrate in the assay buffer, resulting in limited shelf life after first use. In Biotium’s kits, the firefly and Renilla substrates are provided as separate stock solutions that are freshly diluted for every assay, for excellent shelf life with consistent signal over time. The kit features Biotium's Aquaphile™ coelenterazine, a water soluble substrate that can be stored at –20°C with minimal evaporation, unlike methanol solutions of coelenterazine.