This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.

• Highly sensitive and linear flash-type assay
• Substrate is added fresh every assay, for consistent signal over time
• Lyophilized buffer for economical shipping and convenient storage
• Excellent performance and great value compared to other suppliers

This firefly luciferase assay kit is designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. The kit includes assay buffer in lyophilized format for more economical shipping and convenient storage at –20 °C. This is a flash-type luminescence assay that requires signal to be measured immediately after adding working solution to samples. It requires a single tube luminometer or luminescence microplate reader with reagent injectors.

Other commercial luciferase assays require you to dilute all of the firefly substrate in the assay buffer, resulting in limited shelf life after first use. In Biotium’s kits, the substrates are provided as separate stock solutions that are freshly diluted for every assay, for excellent shelf life with consistent signal over time.

Biotium also offers the Steady-Luc HTS Firefly Assay Kit, which is a homogenous glow-type assay with signal half-life of 3 to 5 hours, and the Firefly and Renilla Luciferase Single Tube Assay Kit for sequential measurement of firefly and Renilla luciferase in the same sample.