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Specifications
- Description:5-FAM Conjugate Collagen Hybridizing Peptide (Green)
- Storage Conditions:–20 °C as powder for long term storage. 4 °C after reconstitution in water. Protect from light.
- Solubility:Aqueous Buffers
- Ex/em:494/512 nm
- Cat. No.:103792-902
Specifications
About this item
CHP can specifically bind to denatured collagen.
- Useful in histology, in vivo, and 3D cell culture
- Source: Synthetic
The triple helix is the hallmark protein structure of collagen. During tissue remodeling, the triple helical collagen molecules are degraded by specific proteases (e.g., MMP or cathepsin K) and become unfolded at body temperature. The Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically bind to such denatured collagen strands through hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture). By sharing the structural motif and the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen strands, in a fashion that is similar to a DNA fragment annealing to its complimentary DNA strand during PCR. CHP is an extremely specific probe for unfolded collagen molecules: it has negligible affinity to intact collagen molecules due to the lack of binding sites; it is also inert towards non-specific binding because of its neutral and hydrophilic nature.