HyStem®-HP Hydrogel Kit - The growth factor delivery matrix

• Thiolated Hyaluronic Acid + Gelatin + Heparin Kit
• Ideal for sustained growth factor release
• 12.5 mL kit is for experienced users only

HyStem®-HP Hydrogels - The growth factor delivery matrix. GF's ionically bind with heparin for a more controllable release. Kit includes Thiol-modified hyaluronan and heparin (Heprasil®), PEGDA crosslinker (Extralink®), Thiol-modified gelatin (Gelin-S), and water.

HyStem-HP hydrogel is fully chemically-defined and is ideal for cell applications whereby the slow, continuous release of growth factors is crucial to re-creating a desired microenvironment. The HyStem-HP Hydrogel Kit contains a combination of thiol-modified hyaluronan and a thiol-modified heparin (Heprasil®), thiol-modified denatured collagen (Gelin-S), and thiolreactive crosslinker, PEGDA (Extralink).

The immobilized heparin in the HyStem-HP hydrogel mimics the heparin sulfate proteoglycans normally present in the extracellular matrix. Heparin forms an ionic bond with proteins and protects them from proteolysis and facilitates their slow release into the cell culture medium. This significantly reduces the amount of growth factor required to trigger cell growth or differentiation compared to when growth factors are added directly to the medium.