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- Description:Glycogen phosphorylase assay kit
- Cat. No.:76375-826
- No. of assays/kit:100 assays
Good for detecting glycogen phosphorylase activity.
- Sensitive and Rapid
- Convenient tool for detecting glycogen phosphorylase activity
- The kit can detect as low as 10 mU in a variety of sample types
Glycogen phosphorylase (EC 2.4.1.1) catalyzes the rate-limiting step in glycogenolysis using glycogen and inorganic phosphate to produce glucose-1-phosphate (G1P). In mammals, glycogen phosphorylase is abundant in muscle, liver, and brain tissues. There are two forms of glycogen phosphorylase, namely glycogen phosphorylase a and b forms.
Glycogen phosphorylase a is the highly active form whereas glycogen phosphorylase b has only limited activity. Glycogen phosphorylase is clinically significant enzyme as its mutations are associated with different glycogen storage diseases in muscle and liver. In addition, this enzyme has been suggested as a biomarker for gastric cancer and its inhibition has been tested in treating type 2 diabetes.
BioVision’s Glycogen Phosphorylase Activity Assay Kit is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at 450 nm. The OD 450 nm signal is directly proportional to the glycogen phosphorylase activity. BioVision’s Glycogen Phosphorylase Colorimetric Assay Kit is rapid, sensitive and convenient tool for detecting glycogen phosphorylase activity. The kit can detect as low as 10 µ in a variety of sample types.