LavaLAMP® RNA Component Kit, Loop-mediated Isothermal Amplification (LAMP) of RNA, Biosearch Technologies

Supplier: Lucigen

Certificates

Supplier: Lucigen

About this item

Rapid RNA detection with this fully optimizable RNA LAMP (RT-LAMP) component kit.

Isothermal Amplification: Simplifies RNA detection by enabling use of less complex and lower cost instruments
Lyophilization-ready: All kit components are lyophilization compatible, thus avoiding redesign of assays to remove components known to inhibit lyophilization
Fully Optimizable: Individual kit components enable complete control of reaction formulation to maximize assay performance
Faster RNA Detection: Quicker time to results with less background signals
Equivalent Performance to LavaLAMP™ RNA Master Mix: Assists current users of the master mix product by streamlining additional assay optimization with this kit

Like the related, LavaLAMP® RNA Master Mix, this component kit is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. With these unique features, it is possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) using a single DNA polymerase. The LavaLAMP RNA enzyme first copies the RNA into cDNA, and then loop mediated isothermal amplification takes over to amplify the initial RNA target sequence present in the new cDNA making amplified and detectable target-specific, dsDNA.

The LavaLAMP RNA Component Kit is a deconstruction of the LavaLAMP RNA Master Mix (VWR Cat. No. 76203-026, 76203-028), and as such, when reactions are setup using the initial Experimental reaction conditions outlined in the Component Kit User Manual, the composition of that reaction matches the composition of the recommended initial Master Mix reaction outline in its User Manual. The individual reagent format of this kit, as opposed to the master mix format, enables complete control of each LAMP assay allowing you to carefully identify the best reaction conditions for your specific target and primer set.

To aid in assay development and future lyophilization of LavaLAMP RNA-enzyme based reactions, all components in this kit are compatible with lyophilization. This kit characteristic means that reactions will not have to be reformulated or reoptimized after removal of components that prohibit lyophilization such as glycerol and betaine.

Ordering information: Both LavaLAMP® RNA Component Kits contain: 10X LavaLAMP™ RNA Buffer, LavaLAMP™ RNA Enzyme, Magnesium Sulfate, 100 mM, RNA Positive Control LAMP Primer Mix and RNA Positive Control. The LavaLAMP™ RNA Component Kit with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA (from RNA).

The Green Fluorescent Dye is also available separately.

Caution: For research use only. Not for human or diagnostic use.

Restricted Products: To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

• State issued document with your organization's Federal Tax ID Number
• State issued document with your organization's Resale Tax ID Number
• City or County issued Business License
• State Department of Health Services License
• Any other ID issued by the State that includes the business name & address

* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.