igScript™ one step RT-PCR kit combines two powerful mixtures: igScript™ reverse transcriptase and hot start Taq 2X master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.

• Robust and active for cDNA synthesis at temperatures up to 55 °C
• Highly efficient at producing full-length cDNA from as little as 50 pg of total RNA
• Useful for cDNA synthesis followed by gene expression data validation
• Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product
• Hot start Taq 2X master mix

igScript™ reverse transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42 to 55 °C.

Hot start Taq 2X master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in PCR. The hot start Taq 2X master mix contains standard buffer, chemically-modified hot start Taq polymerase, MgCl₂, dNTPs and stabilizers. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.