T7 Endonuclease I (from Escherichia coli), Intact Genomics

T7 endonuclease I is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells and other applications.

Extremely high purity
Useful to resolve four-way junction or branched DNA, detect/cleave heteroduplex/nicked DNA, and randomly cleave linear DNA for shot-gun cloning
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product
10X T7 endonuclease I reaction buffer

T7 endonuclease I, a stable homodimer of identical 149 amino acid subunits is the product of a recombinant gene in E. coli. Double-stranded breaks (DSBs) generated by CRISPR/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form mismatched DNA. If the mismatched DNA length position is more than 1 bp, T7 endonuclease I can recognize and cleave it. It is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells.

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Source:
E. coli
Enzyme Name:
T7 Endonuclease I
Concentration:
10 U/µl