ZR BAC DNA Miniprep Kit, Zymo Research

About this item

The ZR BAC DNA miniprep kit is for efficient isolation of BAC plasmid DNA and other large plasmids (e.g., PAC) from E. coli cell lysates.

Simple
Rapid
User-friendly
Reliable
No messy precipitations

Unique column design offers zero buffer retention and low-volume (10 µl) elution. It features a modified alkaline lysis protocol together with unique spin column matrix technology to yield high quality plasmid DNA in minutes and color-coded reagents that allow easy visualization and assessment of complete bacterial cell lysis and buffer neutralization.

The innovative Zymo-Spin™ IC-XL columns are optimized for high yield endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA miniprep kit is well suited for sequencing, PCR, endonuclease digestion, and more.

Delivery: The kit includes P1 buffer, P2 buffer, P3 buffer, endo-wash buffer, plasmid wash buffer (concentrate), DNA elution buffer, Zymo-Spin™ IC-XL columns, collection tubes and instruction manual.

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Specifications

Method / Format:
Column (silica-based)
Prep size:
Mini
Refine target molecule:
Larger vectors (Cosmid, BAC, PAC, Fosmid)
Sample type:
Bacteria
Target molecule:
Plasmid
Description:
ZR BAC DNA miniprep kit
Yield:
Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture.