Lympholyte®-M Cell Separation Media, Cedarlane

Lympholyte®-M is a density separation medium specifically designed for the isolation of viable lymphocytes from murine lymphoid cell suspensions.

• Composition: Polysucrose 400 and sodium diatrizoate
• Density: 1.0875 ±0.0010 g/cm³ at 22 °C
• pH: 6.9 ±0.3
• Viability/Purity: Recovery of viable lymphocytes >70%

Lympholyte®-M can be utilized in a simple protocol to eliminate erythrocytes, dead cells and debris from murine spleen, lymph node, thymus and bone marrow suspensions. The resulting cell population demonstrates a high and nonselective recovery of viable lymphocytes that are suitable for use as target cells in cytotoxicity, FACS assays, and in in vivo and in vitro functional studies.

Applications include the removal of dead cells in sequential cytotoxicity studies, the removal of erythrocytes, dead cells and debris from other murine tissue
suspensions including liver and lung, the harvesting of viable cells and removal of dead cells and debris from various clone cell and hybridoma cell lines and the isolation of murine nuclear epidermal cells.