ELISA Kit, Quest 5-hmC DNA, Zymo Research

About this item

The Quest 5-hmC DNA ELISA kit is both sensitive and specific and can be used to accurately detect 5-hmC DNA in a variety of samples. The kit is compatible with a wide range of input DNA including intact vertebrate, plant, and microbial genomic DNA, as well as enzyme-digested and mechanically sheared fragments.

Ideal for global 5-hmC quantitation and high throughput screening
Streamlined workflow can be completed in 3 hrs

The control DNA set included with this kit has been calibrated to accurately quantify the percent 5-hmC in sample DNA by use of a standard curve. Also, the fast, streamlined workflow is ideal when analyzing/screening large numbers of samples.

This protocol is optimized for 100 ng of input DNA/well. Compatible with DNA in the range of 20 - 200 ng/well.

Delivery: The kit includes coating buffer (15 ml), 10X ELISA buffer (30 ml), Anti-DNA HRP antibody 100X (100 µl), HRP Developer (15 ml), Control A (100 ng/µl) (40 µl), Control B (100 ng/µl) (40 µl), Control C (100 ng/µl) (40 µl), Control D (100 ng/µl) (40 µl), Control E (100 ng/µl) (40 µl), Anti-5-hydroxymethylcytosine polyclonal antibody (25 µg), 96-Well ELISA plate (12×8 well-strips).