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Glucose Uptake-Glo™ Assay, Promega
Glucose Uptake-Glo™ Assay, Promega
  76201-554
 :  
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Glucose Uptake-Glo™ Assay, Promega
  76201-554
 :  J1341
 :  
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  • Description:
    Glucose Uptake-Glo™ Assay Kit
  • Size:
    1 Kit
  • Environmentally Preferable:
  • Cat. No.:
    76201-554

 

 

A simple, non-radioactive assay used to measure glucose uptake inside cells.

  • Homogeneous protocol
  • Achieve sensitivity with broad linearity
  • Reliable and reproducible results

Glucose metabolism is a key process in many organisms. A lack of insulin-stimulated glucose uptake is associated with type 2 diabetes, while high glucose uptake is a sign of the high glycolytic rates associated with cancer. Measuring glucose uptake can determine the effects of various treatments for diabetes and cancer.

The gold standard method of assaying glucose uptake relies on detection of radio-labelled glucose analogs. Alternative colorimetric and fluorometric glucose uptake assays often lack the sensitivity and robustness needed to reliably measure glucose uptake in cells.

 : The system contains sufficient reagents to perform 50 reactions in 96-well plates (50 µl of sample, 25 µl of stop buffer, 25 µl of neutralization buffer, 100 µl of 2DG6P detection reagent). It includes luciferase reagent (5 ml), stop buffer (15 ml), neutralization buffer (15 ml), NADP+ (20mM) (50 μl), glucose-6-phosphate dehydrogenase (G6PDH) (125 μl), reductase (25 μl), reductase substrate (55 μl), 2-deoxyglucose (2DG, 100mM) (250 μl), 2DG6P standard (1mM) (50 μl). Store the kit at –65 °C.
 : For research use only. Not for use in diagnostic procedures.