IncuCyte® Caspase-3/7 red apoptosis assay reagent couples the activated caspase-3/7 recognition motif (DEVD) to NucView™488, a DNA intercalating dye to enable quantification of apoptosis over time.

• Inert
• Requires no wash, mix and read protocol

It is specially formulated for use in the IncuCyte® live-cell analysis system and can be added directly to tissue culture wells. It is non-perturbing to cell growth and morphology. When added to tissue culture medium, this inert, non-fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7 resulting in the release of the DNA dye and red fluorescent staining of nuclear DNA. Kinetic activation of caspase-3/7 can be monitored morphologically using live cell imaging and quantified using the IncuCyte® basic analyzer.