RedAlert™ stain is used to visualize protein transferred to membranes prior to western blot analysis or protein sequencing by Edman degradation. RedAlert stain binds reversibly to proteins on nitrocellulose and PVDF membranes and can also stain proteins bound to cellulose acetate. This stain binds positively charged amino groups and nonpolar regions of proteins, resulting in reddish-pink bands.

Designed to verify transfer of proteins before immunological detection on nitrocellulose or PVDF membranes or to detect blood serum proteins on cellulose acetate media.