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- Description:His Capture Kit, type 2
- Cat. No.:103568-410
- Storage temperature:+2…+8 °C
- No. of reactions:16 immobilizations, 1600 regenerations
Reagents for capture of histidine-tagged molecules as ligands in biomolecular interaction analysis. Designed for use with Biacore 8K+, Biacore 8K (larger kit component volumes than in His Capture Kit) but can be used with all other Biacore systems.
- Enables capture of histidine-tagged ligands
- Physiological conditions can be used during the coupling
- Optimized and verified assay minimizing assay development
It is designed for capture of histidine-tagged proteins to an anti-histidine antibody and provides an alternative to capture on nickel-chelated nitrilotriacetic (NTA) groups.
The anti-histidine antibody is a monoclonal antibody directed against polyhistidine tags in the C- or N-terminus of a protein. Depending on the nature of the interacting molecules and the micro-environment around the histidine-tag, the affinity of the capture antibodies will vary.