For the quantification of 15(S)-HETE in urine, plasma, serum, whole blood, and other sample matrices.

• Detect 15(S)-HETE, a product of 15-lipoxygenase treatment of arachidonic acid
• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure 15(S)-HETE levels down to 170 pg/ml
• Read: Colorimetric at 405 to 420 nm

15(S)-HETE is produced from arachidonic acid by the enzyme 15-LO. In humans it is formed primarily in the respiratory epithelium, leukocytes, and reticulocytes. It may contribute to allergic rhinitis, has anti-inflammatory properties, and acts as a vasoconstrictor.

This assay is very specific for 15(S)-HETE, showing very low crossreactivity to other HETEs.