This kit is used for the measurement of 13,14-dihydro-15-keto prostaglandin F₂α in plasma.

• Detect 13,14-dihydro-15-keto PGF_2α, a stable metabolite of PGF_2α
• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure 13,14-dihydro-15-keto PGF_2α levels down to 16.3 pg/ml
• Reads colorimetric at 405 to 420 nm

PGF_2α is rapidly metabolized to 13,14-dihydro-15-keto PGF_2α in vivo, by the enzymes 15-hydroxy PG dehydrogenase and D13-reductase. Measurement of 13,14-dihydro-15-keto PGF_2α in plasma can be used as a marker of the in vivo production of PGF_2α.

13,14-dihydro-15-keto PGF_2α ELISA is a competitive assay that provides measurements of 13,14-dihydro-15-keto PGF_2α within the range of 2.3 to 5000 pg/ml. The assay has been validated for the measurement of 13,14-dihydro-15-keto PGF_2α in plasma, a common matrix for measurement of this PGF_2α metabolite.