Iscove's modified dulbecco's medium (IMDM) contains 4 mM L-glutamine, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate.

• pH: 7.0 to 7.4
• Osmolality: 235 to 309 mOsm/kg
• Sterility testing: Pass
• Endotoxin: ≤0.5 EU/ml
• Mycoplasma: Pass
• Growth promotion: Pass

Iscove's modified dulbecco's medium (IMDM) reduced level of sodium bicarbonate (NaHCO₃, 1.5 g/L) is intended for use in 5% CO₂ in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO₂.