Human Recombinant VEGF165 (from HEK293 Cells) His Tag

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Specifications

Conjugation:
Unconjugated
Protein Function:
Cytokines and Growth Factors
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Human
Tag sequence:
His Tag
Storage Conditions:
–20 °C
Endotoxin Content:
Less than 1.0 EU per μg by the LAL method.
Biological Activity:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human VEGF R1&VEGF R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with a linear range of 0.2-4 ng/mL (QC tested).; Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165 Protein, His Tag (Cat. No. VE5-H5248) with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protease-free:
Y
Animal-Free:
Y
Protein Synonyms:
RP1-261G23.1|MVCD1|MGC70609|VEGFA|VPF
UniProtKB:
P15692-4
Protein/Peptide Name:
VEGF165
Purity:
>90% as determined by SDS-PAGE.
Molecular Weight:
21.1 kDa
Endotoxin Level:
Low
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Shipping Temperature:
Powder,RT