Specifications

• Conjugation:
  Unconjugated
• Protein Function:
  Immune Checkpoint Proteins
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  Fc Tag
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.; Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4 (Cat. No. CT4-H82E4) with a linear range of 50-500 ng/mL (Routinely tested).; Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 2 μg/mL ( 100 μl/well ) can bind Human CTLA-4, Strep Tag (Cat. No. CT4-H5284) with a linear range of 1-20 ng/mL (QC tested).; Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, His Tag, Fc Tag (Cat. No. PD1-H82F3) with a linear range of 0.02-1.25 μg/mL (Routinely tested).; FACS assay shows that Human B7-1, Fc Tag (HPLC-verified)(Cat. No. B71-H5259) can bind to 293T cells overexpressing human CTLA4 protein. The concentration of Human B7-1 is 1 μg/ml (Routinely tested).
• Protease-free:
  Y
• Animal-Free:
  Y
• Protein Synonyms:
  B7-1|B7.1|CD28LG|CD28LG1|LAB7|BB1|CD80|B7
• UniProtKB:
  P33681
• Protein/Peptide Name:
  B7-1
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular Weight:
  50 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  Powder,RT