- Antibody Type:Primary
- Antigen Symbol:SHISA3
- Clonality:Polyclonal
- ELISA:Yes
- Host:Rabbit
- Western Blot:Yes
- Size:100 µg
- Storage Temperature:Store vial at –20 °C. Aliquot contents and freeze at –20 °C. Centrifuge product if not completely clear after standing at room temperature. Stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
- Shipping Temperature:Dry Ice
- Purification:Affinity purified
- Cat. No.:75935-368
- Supplier no.:600-401-EJ1
SHISA3 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.
Anti-SHISA3 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 26 kDa in Western Blots of specific cell lysates and tissues.
Type: Primary
Antigen: SHISA3
Clonality: Polyclonal
Clone:
Conjugation:
Epitope:
Host: Rabbit
Isotype:
Reactivity: