1.0 M HEPES pH 7.2

About this item

HEPES is a zwitterionic organic chemical buffering agent.

HEPES is a useful buffer for pH's ranging from 6.8-8.2.
This makes HEPES ideal for cell culturing because of its usefulness in maintaining pH changes in presence of carbon dioxide production from cellular respiration.
1.0 M HEPES pH 7.2 is ideal for investigators in Molecular Biology, Cell Biology, and Immunology researchers.

HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. HEPES is like water in that its dissociation decreases as the temperature decreases. This makes HEPES a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

Application Note: 1.0 M HEPES is a concentrated stock solution and should be diluted appropriately with molecular biology grade water or equivalent to its final working concentration. This buffer consists of 1.0 M HEPES at a pH of 7.2 and was prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionized water.

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