Mouse Relaxin 1 AccuSignal ELISA Kit

• Natural and recombinant mouse Relaxin 1.
• There is no detectable cross-reactivity with other relevant proteins.
• Expression system for standard: E.coli; Immunogen sequence: R23-R54(B)&E161-C185(A)

Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix(cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/ml mouse Relaxin 1 standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each mouse Relaxin 1 standard solution and each sample is measured in duplicate.