Order Entry
United States
ContactUsLinkComponent
 
Mouse P-Selectin ELISA Kit, Rockland Immunochemicals
  76237-182
 :  
Mouse P-Selectin ELISA Kit, Rockland Immunochemicals
  76237-182
 :  KOA0302
 :  

 

  • Assay Type:
    Sandwich
  • Primary antibody reactivity:
    Mouse
  • Description:
    Mouse P-Selectin ELISA Kit
  • Size:
    1 Kit
  • Shipping Temperature:
    Wet Ice
  • Cat. No.:
    76237-182

 

 

Mouse P-Selectin AccuSignal ELISA Kit

  • Natural and recombinant mouse sP-Selectin.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: NSO; Immunogen sequence: W42-A709

P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse P-Selectin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(citrate) to each empty well. It is recommended that each mouse P-Selectin standard solution and each sample be measured in duplicate.