AccuSignal Rabbit FITC-DAB (ABC) Immunohistochemistry Detection Kit

• Immunohistochemistry Detection Kit
• unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, fluorescein-conjugated streptavidin, and chromogen substrate

Rabbit IgG Specific Fluorescein Steptavidin-Biotin Complex Immunohistochemistry Detection Kit is a streptavidin-biotin immuno-enzymatic antigen detection system. This system involves the sequential incubation of the sample with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, fluorescein-conjugated streptavidin, and chromogen substrate. Rabbit Specific FITC/DAB (ABC) Detection Kit includes reagents specifically designed for the use in the analysis of antigens in IHC and other immunodetection assays. The provided protocol includes steps for high sesitivity analysis with low backgound using normal goat serum blocking reagent, biotinylated secondary antibody, and a FITC-conjugated streptavadin.

This kit contains concentrated products and will need dilution. See kit insert for details. Useful for Immunohistochemistry. IHC(P): 1:50-200, IHC(F): 1:50-200, ICC: 1:50-200. Optimal dilutions should be determined by end users.