Mouse Recombinant PDGF-BB (from E. coli)

Recombinant Mouse PDGF-BB control protein

Control Protein
PCA Assays
ImmunoBlot

Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant mouse PDGF-BB is a non-glycosylated, disulfide-linked homodimer, containing two 109 amino acids chains, with a total molecular weight of 24.6 kDa.

Platelet Derived Growth Factor-BB Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-BB in immunological assays.

Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Mouse
Storage Conditions:
Store vial at 4 °C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at –20 °C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
Endotoxin Content:
Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.
Biological Activity:
The activity is measured by the dose-dependent proliferation in mouse 3T3 fibroblasts and is typically 1-2 ng/mL.
Gene ID:
P31240
Reconstitution Instructions:
Restore with deionized water (or equivalent); 1.0 mL
Protein Synonyms:
Proto-oncogene c-Sis|Platelet-derived growth factor B chain|Platelet-derived growth factor beta polypeptide|PDGF-2
Protein/Peptide Name:
PDGF-BB
Purity:
>98%
Formulation:
Lyophilized
Shipping Temperature:
Ambient