Human Recombinant PDGF-AA (from E. coli)
Supplier: Rockland Immunochemical
Certificates
About this item
Recombinant Human PDGF-AA control protein
- Control Protein
- PCA Assays
- ImmunoBlot
Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant human PDGF-AA is a non-glycosylated disulfide-linked homodimer, containing two 125 amino acid chains, with a total molecular weight of 28.5 kDa.
Platelet Derived Growth Factor-AA Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-AA in immunological assays.
Specifications
- Protein/Peptide Type:Recombinant
- Source:E. coli
- Species:Human
- Storage Conditions:Store vial at 4 °C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at –20 °C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Endotoxin Content:Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.
- Biological Activity:The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically 3-5 ng/mL.
- Gene ID:P04085
- Reconstitution Instructions:Restore with deionized water (or equivalent); 1.0 mL
- Animal-Free:Y
- Protein Synonyms:Platelet-derived growth factor alpha polypeptide|Platelet-derived growth factor A chain|PDGF-1
- Protein/Peptide Name:PDGF-AA
- Purity:>97%
- Formulation:Lyophilized
- Shipping Temperature:Ambient
Specifications
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