The NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina provides a fragmentation system that is a fast and reliable solution for DNA fragmentation and library construction.

• Perform fragmentation, end repair and dA-tailing with a single enzyme mix
• Experience reliable fragmentation with a single protocol, regardless of DNA input amount or GC content
• Generate high yields with increased reaction efficiencies and minimized sample loss
• Use with DNA in standard buffers (TE, Tris-HCl) and water
• Save time with a streamlined workflow: ~2.5 hours, with <15 minutes hands-on time
• Vary incubation time to generate a wide range of insert sizes

A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 to 500 ng), or GC content.